Gene structure and transcription:

You have cloned a new bacterial gene encoding enzyme X, sequenced the DNA, and deduced the promoter and coding region as shown above. You prepare a gene construct with the deletion above; the deletion does not remove any of the promoter nor does it alter the open reading frame. When both the control and deletion genes are expressed in E. coli and assayed for enzyme X, you find that only the control shows enzyme activity. WHY? How can you test your hypothesis?