Q1. Build the relations for the dependence of the melting temperature of a 30 bp DNA duplex on the G-C content. For a sequence of DNA chains where we vary the number of G-C pairs, plot how the Tm changes as a function of f, the fraction of G-C pairs, ranging from 0.33 (that is, 10 G-C pairs) to 0.67 (that is, 30 G-C) pairs.
From the surveillance, what other factors (other than the G-C content) seem to control Tm?
Q2. Supposing we have two actin filament 10 microns long joined by 10 myosin motors, how long would be needed to move the two filaments past each other 5 microns? How much energy is needed?