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What is known about the Get pathway for membrane protein insertion?

For Figure 1D, describe the experimental set-up and how the results indicate the Get3 can take Sec22 from Get4/5.

How do we know that Get3 transport to the ER is dependent on Get1/Get2 (Fig. 1E)?

In Figure 2A, which lane indicates that Sgt2 is required for association of Get5 with the rest of the protein complex? Explain your answer.

The experimenters induce a mutation in Sgt2 that reduces binding of Hsps to Sgt2. Does this affect the binding of Sgt2 and Sec22?

 

 

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