Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Biology Expert

Ansswer the following problems:

1) prepare down the factors contribute to an efficient ligation reaction?            

2) State the start codon in both formats of the nucleic acid.                

3) Which enzyme might be considered as the in vitro version of RNAP?        

4) describe the use of digoxigenin in rDNA technology.                    

5) prepare down the applications of alkaline phosphatase in rDNA technology.            

6) What vectors are used for the design of RNA probes and how are they unique?        

7) Describe the application of DNA homology experiments to determine the relatedness between two species.                                            

8) Whole human genome is about 3 x 109 bp long. Eco RI cuts on average every 4.1 kb. The exact probability of having any given DNA sequence in the library, P is given by: N = ln(1 -P) / ln(1 - f). f is the fractional proportion of the genome in a single recombinant and N is the necessary number of recombinants. How large a library will you need in order to have a 99% probability of finding a desired sequence represented in a library created by digestion with a 6-cutter?                            

9) prepare down the steps involved in Northern Blotting.

Biology, Academics

  • Category:- Biology
  • Reference No.:- M94427

Have any Question? 


Related Questions in Biology

Trace the pathway of the blood through the heart and

Trace the pathway of the blood through the heart and differentiate between pulmonary, systemic, and cardiac circulation.

What is the difference between cis-acting elements and

What is the difference between cis-acting elements and trans-acting factors in the regulation of DNA?

Trace the pathway of the blood through the heart and

Trace the pathway of the blood through the heart and differentiate between pulmonary, systemic, and cardiac circulation.

1 define partial pressure and how it is calculated2

1. Define partial pressure and how it is calculated. 2. Compare the partial pressure at sea level and at the top of Mt. Everest. Explain why, if oxygen is still at the same concentration in both locations, the partial pr ...

Select a current news item pertaining to an ethical

Select a current news item pertaining to an ethical situation in the Middle East that has arisen in a business or non-profit organization. Describe the situation and the organization. Determine what made or makes this si ...

How does presence of both high and low affinity operators

How does presence of both high and low affinity operators allow for coordination of DNA repair, recombination bypass, and translesion synthesis in response to varying levels of DNA damage?

Assignmentshort answeressay write your answers and submit

Assignment Short Answer ESSAY. Write your answers and submit assignment via the attachment. Please be specific and use examples from the textbook when appropriate 1) What 4 factors do you think should be taken into consi ...

A bond with a par value of 1000 and a semi annual coupon

A bond with a par value of $1000 and a semi annual coupon has a nominal yield to maturity of 5.3% and a current price of $920 . if the bond has 6 years to maturity, what is its current yield?

What would be the expected genotypes and phenotypes of the

What would be the expected genotypes and phenotypes of the children and their relative frequencies from the following crosses a) NN X nn b) Nn x nn c) Nn x Nn d) nn x nn Where n represent gene for albinism and N represen ...

Select at least 2 cell structures what hypothesis could you

Select at least 2 cell structures. What hypothesis could you form to explain what would happen to the cell or the organism if each cell structure were damaged or destroyed?

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Section onea in an atwood machine suppose two objects of

SECTION ONE (a) In an Atwood Machine, suppose two objects of unequal mass are hung vertically over a frictionless

Part 1you work in hr for a company that operates a factory

Part 1: You work in HR for a company that operates a factory manufacturing fiberglass. There are several hundred empl

Details on advanced accounting paperthis paper is intended

DETAILS ON ADVANCED ACCOUNTING PAPER This paper is intended for students to apply the theoretical knowledge around ac

Create a provider database and related reports and queries

Create a provider database and related reports and queries to capture contact information for potential PC component pro

Describe what you learned about the impact of economic

Describe what you learned about the impact of economic, social, and demographic trends affecting the US labor environmen