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Explain the Determination of Hydrolysis

Hydrolysis of calcium pantothenate in acidic medium produces α,γ-dihydroxy-β,β-dimethylbutyrolactone, which reacts with hydroxylamine in the presence of alkalis to produce the corresponding hydroxamic acid. After addition of ferric chloride solution, the purple colour produced is measured at 500 nm.

The microbiological assay involves the titrimetric determination of lactic acid produced by Lactobacillus arabinosus ATCC 8014.

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