Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Biology Expert

describe Isolation of RNA

To isolate RNA you frequently use a protein denaturant solution of guanidinum salts. These chaotropic explanations denature by disrupting H- bonding. The solutions as well contains a reducing agent to denature disulfide bonds. The final composition of the solution is: 4 guanidine isthiocyanate (GITC), 25mM sodium citrate, pH 7.0 , 0.5%(w/v)SDS, 100mM β-mercaptoethanol. You have guanidine isothiocyanate powder (mol.Wt.118.16), 1 M NaCitrate, pH 7.0, powder SDS and b-mercaptoethanol (density 1.12 g/mL, mol. Wt.78.1). Exactly how would you make up 100 mL of this solution?

Biology, Academics

  • Category:- Biology
  • Reference No.:- M916511

Have any Question? 


Related Questions in Biology

Lets say that robert baratheon despite being king was

Let's say that Robert Baratheon, despite being king, was unlucky enough to be heterozygous from Huntington's disease and heterozygous polycystic kidney disease-2 (caused by a mutation in the Polycystin-2 gene). However, ...

Mrs smith is 50 years of age and is nervous about going

Mrs. Smith is 50 years of age and is nervous about going through menopause. How would you explain the process to her? What effects do estrogen and progesterone have on the vaginal mucosa? How does the effect of estrogen ...

How does human evolution or natural selection relate to the

How does human evolution or natural selection relate to the susceptibility of disease?

1 how can it be that a double mutant embryo that lacks

1. How can it be that a double mutant embryo that lacks maternally encoded Hunchback and Nanos protein is completely viable and fertile? 2. The terminal regions of the Drosophila embryo involve another maternally pathway ...

Over half of the bodys glucose is metabolized in the brain

Over half of the body's glucose is metabolized in the brain. Very few of the alternate fuels are used in the brain, but are commonly used by other parts of the body. Muscles increase their use of glucose with increasing ...

Which animals have a built in thermostat to maintain

Which animals have a built in thermostat to maintain constant body temperature?

We all see animals in our day to day lives choose a

We all see animals in our day to day lives. Choose a behavior you saw exhibited by an animal. Explain your thoughts on the potential proximate and ultimate causes of the behavior.

The lac operator binds the lac repressor the dna sequence

The lac operator binds the lac repressor. The DNA sequence of the lac operator shares a characteristic with the DNA sequences of the binding sites of many restriction enzymes. What is this common characteristic?

How does sexual reproduction benefit the offspring in

How does sexual reproduction benefit the offspring in terms of genetic variation? Provide an example

Climate of Climate of Doubt:  www.pbs.org/wgbh/frontline/film/climate-of-doubt/ this

Climate of Doubt:  www.pbs.org/wgbh/frontline/film/climate-of-doubt/ this video is about 1 hour watch.  Anyone who answer this question skill this video up to 25min then watch the rest. 1. Who is Bob Inglis and what happ ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

WalMart Identification of theory and critical discussion

Drawing on the prescribed text and/or relevant academic literature, produce a paper which discusses the nature of group

Section onea in an atwood machine suppose two objects of

SECTION ONE (a) In an Atwood Machine, suppose two objects of unequal mass are hung vertically over a frictionless

Part 1you work in hr for a company that operates a factory

Part 1: You work in HR for a company that operates a factory manufacturing fiberglass. There are several hundred empl

Details on advanced accounting paperthis paper is intended

DETAILS ON ADVANCED ACCOUNTING PAPER This paper is intended for students to apply the theoretical knowledge around ac

Create a provider database and related reports and queries

Create a provider database and related reports and queries to capture contact information for potential PC component pro