E. coli were transformed with 50 ng of plasmid dna is a, Ask an Expert

Academics

E. coli were transformed with 50 ng of plasmid DNA is a total volume of 500 µl, and 100 µl of the transformation reaction was plated on a LB amp agar plate. After overnight incubation at 37C.

Count the colonies and calculate the transformation efficiency for this experiment.

View complete question

Biology, Academics

Category:- Biology
Reference No.:- M9398632

Have any Question?Write your Review or question?

Related Questions in Biology

Quesiton synthetic chromosomes transcriptomes and patents

Quesiton: "Synthetic chromosomes, Transcriptomes, and Patents on BRCA genes" For your primary post, please respond to one of the following three topics with a post of at least 125 words that addresses each point given in ...

The allele for hitchhikers thumb is recessive to the

The allele for hitchhiker's thumb is recessive to the dominant straight thumb. In a population of 500 students, 25% have hitchhiker's thumb. How many students would you expect to be homozygous dominant for the trait?

Question overview although it may sound like science 2520

Question: Overview: Although it may sound like science 2520, DNA and RNA technologies are already used in products today. In this discussion you will examine one specific product, organism, or technology in your initial ...

1 identify the maternal homolog for both chromosome 1 and 2

1.) Identify the maternal "homolog" for both Chromosome 1 and 2 and then the paternal homologs. Arrange these chromosomes as you would expect to find them during G 1 of interphase. What is the haploid number of our ce ...

How do specimens move when put under the dissecting

How do specimens move when put under the dissecting microscope?

The ch50 assay is a traditional clinical assay that

The CH50 assay is a traditional clinical assay that measures the function of complement components. In a CH50 assay patient blood is drawn and serum separated from it (serum has no cells). Patient serum is mixed with ant ...

What is the process of turning polynucleotides into

What is the process of turning polynucleotides into proteins?

Question - gene cloning a please write the steps to clone

Question - Gene Cloning a) Please write the steps to clone the protease gene from Bacillus strain whose genome sequence is not known. b) Express the protease gene to obtain the enzyme in high yield, please plan your prot ...

If you put the letter e on a slide underneath a light

If you put the letter "e" on a slide underneath a light microscope and physically move it from left to right, which way does the letter appear to move when viewed through the ocular lens? What if you physically move the ...

A suspension is formed from uniform particles of solid of

A suspension is formed from uniform particles of solid, of diameter 10 Mm, suspended in a solvent. What is the best description of this system?

4,153,160 Questions Asked
13,132 Experts
2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Recent Questions

Ask Biology Expert