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During the separation of the different photosynthetic pigments of spinach by paper chromatography, how did you know which of the pigments was the least polar?

  • It travelled the farthest on the paper (was the first pigment from top to bottom)
  • Travelled the least (was the lowermost band of pigment on the paper from top to bottom)
  • Did not stick to the paper very well
  • Was difficult to extract out of the paper

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