Q1. Design pentamer (5 nucleotide) primers that would amplify the entire DNA sequence shown here.

            5'-ATTCGGATTACATCGGCATTACCGATTTAAAGCCCTTAACG-3'

            3'-TAAGCCTAATGTAGCCGTAATGGCTAAATTTCGGGAATTGC-5'

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Q2. Draw a restriction map based on the fragments resulting from the following restriction digestions of a 3 kb plasmid

            Enzyme                        Fragment size (kb)

            EcoRI                          1.0; 2.0

            BamHI                         1.3; 1.7

            EcoRI + BamHI           0.4; 0.6; 0.7; 1.3