Dr. j. walker meticulously prepares an sirna 22 bp duplex, Ask an Expert

Academics

Dr. J. Walker meticulously prepares an siRNA (22 bp duplex) and transfected cultured cells. He finds that the target gene mRNA is indeed knocked down, but so are 5 other non target mRNAs. The effect is sequence-specific and not an artifact. Rationalize this finding.

View complete question

Biology, Academics

Category:- Biology
Reference No.:- M998385

Have any Question?Write your Review or question?

Related Questions in Biology

The current scientific defination of standard temp and

The current scientific defination of standard temp and pressure (STP) is: 0 degrees C and 1.00 bar pressure of a gas, using ideal gas law calculate the volume In L occupied by 1 mol of an ideal gas under these condition( ...

Is it possible to increase the temperature of a gas in a

Is it possible to increase the temperature of a gas in a cylinder without any energy addition as heat? Explain your answer using the first law of thermodynamics.

Select two of the mechanisms of evolutionary change

Select two of the mechanisms of evolutionary change (mutation, gene flow, genetic drift or non-random breeding) and explain what each is and how each can lead to evolutionary change(change in genetic compositions within ...

2croh3nbspnbsp3br2 10oh-6br-nbspnbsp2cro42- 8h2oin the

2Cr(OH) 3 + 3Br 2 + 10OH - 6Br - + 2CrO 4 2- + 8H 2 O In the above redox reaction, use oxidation numbers to identify the element oxidized, the element reduced, the oxidizing agent and the reducing agent.

You take a small section of leaf and view it underneath a

You take a small section of leaf and view it underneath a microscope. You count 25 stomata. Now you want to calculate how many stomata would be in a leaf the size of a penny. a) Calculate the area of the view of view (A= ...

Krebs cycletca cyclewhat are the reactantswhat are the

Krebs Cycle/TCA cycle What are the reactants? What are the products? What is the net ATP gain? Where does it take place in prokaryotes vs eukaryotes?

Trace the flow of carbon within the process of

Trace the flow of carbon within the process of photosynthesis. Be sure to include the following terms in your description: Glucose.NADPH,ATP, Calvin cycle, RUBISCO,CO2.

Question summaryfor readability please be sure to

Question: Summary For readability, please be sure to double-space your assignments. A new wild strain of nopal cactus has been identified as having remarkable promise to solve the international dietary manganese deficit ...

Which are the logical steps proposed by margulis that would

Which are the logical steps proposed by Margulis that would help explain why the accumulation of oxygen in the atmosphere led to the evolution of mitochondria.

What problems does this traditional classification scheme

What problems does this traditional classification scheme of protists create in our understanding of the evolutionary relationships that exist between protists?

4,153,160 Questions Asked
13,132 Experts
2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Recent Questions

Ask Biology Expert