Given this DNA template sequence of a gene encoding mRNA in E.coli:
5'-ATG TAG TTA TCC AAC CCT CTT AAA CAT GCA CCT CCT TAC TCA AGC AGG CAT ACG-3'
...One of the tRNA genes in E.coli was mutated at the anticodon site. The anticodon was changed from 5'-CUU-3' to 5'-CUC-3'.
1.) What does this mutation do to the protein sequence (full template) from above?
2.) What is the total charge of this protein?
3.) What do you think the consequences are for this type of mutation in e.coli. Will the e.coli survive? Explain your answer in detail, please!