1) Specify and describe the various techniques of sterilization.

2) describe compositions of the culture media? Also highlight the significance of each of the compositions.

3) prepare a brief note over the Callus subculture and organogenesis.

4) describe the method for meristem tip culture for producing the virus free plants with the help of an ex.

5) describe some of the benefits of the invitro regeneration of the plants from the cotyledons of leguminous species? Describe the steps that are included in it.

6) List out the different methods for eliminating the viral contaminants from the plant micro-propagation?

7) describe the term cryopreservation? Describe the steps that are involved in the preparation of the materials for the purpose of cryopreservation.

8) Distinguish between the cytosenescence and cytoquiescence? describe the primary steps in de-differentiation and re-differentiation of the plant cells?

9) describe the applications of plant tissue culture within the field of agriculture, horticulture and forestry?

10) Specify and describe different methods for improving the quality of crop?