problem 1:

describe in brief the describable properties and functional organization of the plasmid cloning vehicles.

problem 2:

Illustrate out the structure and methodology of construction of any two pBR 322 system.

problem 3:

Critically discuss the use of the Lambda phage as vector for coning eukaryotic DNA.

problem 4:

Discuss the role of M13 vectors in DNA sequencing.

problem 5:

Discuss how the cloned gene of interest is transferred to plants by Agrobacterium Tumefaciens.

problem 6:

Illustrate out the different animal viruses which are employed in gene cloning with their particular features.