describe in brief the describable properties and functional organization of the plasmid cloning vehicles.
Illustrate out the structure and methodology of construction of any two pBR 322 system.
Critically discuss the use of the Lambda phage as vector for coning eukaryotic DNA.
Discuss the role of M13 vectors in DNA sequencing.
Discuss how the cloned gene of interest is transferred to plants by Agrobacterium Tumefaciens.
Illustrate out the different animal viruses which are employed in gene cloning with their particular features.