One mL of yogurt was added to 99 ml of sterile diluent. Decimal (1/10) dilutions were then made, and one-tenth ml was plated in duplicate on Plate Count Agar. After incubation, the following colony counts were made:
- Initial dilution TNTC,TNTC
- First 1/10 dilution TNTC, TNTC
- Second 1/10 dilution 412, 422
- Third 1/10 dilution 54, 56
- Fourth 1/10 dilution 6,4
Dilution Colony count Using the data, calculate the number of CFUs per gram of the yogurt (1mL of yogurt = 0.4g).