The following kinetic data were obtained for an enzyme in the absence and in the presence of an inhibitor at a 5 mM concentration. You can assume that the same amount of enzyme was present in each assay, [ET]
|
Substrate
|
Rate
|
Rate (+inhibitor)
|
|
mM
|
mM/min
|
mM/Min
|
|
1
|
1.3
|
0.8
|
|
2
|
2
|
1.2
|
|
3
|
2.8
|
1.7
|
|
8
|
3.6
|
2.2
|
|
12
|
4
|
2.4
|
Determine the Km and Vmax values for the enzyme in the presence and presence of the inhibitor. (Show your graphical results!) Report Km or Vmax values in mM or mM/min as appropriate, and indicate which type of inhibition is demonstrated by the inhibitor? You MUST explain your reasoning