Question: Determine the final guaiacol concentrationg (answers in units of guaiacol) of the following enzyme reaction solutions listed in the table below. Keep in mind that each reaction solution has a total volume of 2000 microliters.
Tube |
0.05M Buffer |
20mM Hydrogen Peroxide |
80 mM Guaiacol |
0.5 units/ml Peroxidase |
Total Volume |
Tube 1 |
1000microliters |
400 microliters |
350 microliters |
250 microliters |
2000 ul |
Tube 2 |
800microliters |
400 microliters |
550 microliters |
250 microliters |
2000 ul |
Tube 3 |
600microliters |
400 microliters |
750 microliters |
250 microliters |
2000 ul |