Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Biology Expert

1. You isolate a Gram positive alkalinophile that grows at a pH = 10. At this external pH, the intracellular pH is maintained at 7.3. Using the equation proton motive force (pmf), determine the contributions of the electrical and chemical energy components to the pmf in this bacterium.

2. You are studying the type VI secretion system in Pseudomonas aeruginosa. You analyze the genome and find an operon comprised of 5 genes that you believe is involved building the secretion apparatus. You name the genes tssA, tssB, tssC, tssD, and tssE based simply on their order. You believe that tssA(the first gene in the operon) encodes the crucial component of the secretion apparatus. Design an experiment to make a knockout of tssA. How will you determine if the mutation in tssA is a polar mutation?

3. You obtain a clinical isolate of the Gram positive pathogen Clostridium difficile that is highly resistant to vancomycin. This is a significant health concern as vancomycin is a ‘last line of defense' for treatment of C. difficile infections. You notice that all of the clinical isolates were taken from patients suffering from a multispecies infection, including a vancomycin resistant strain of E. faecalis. What coulddescribe this? State your hypothesis and develop experiments to test it.

4. You treat 107Pseudomonas aeruginosa growing planktonically and as a biofilm with high levels of the antibiotic tobramycin (10 micrograms/ml) for 2 hours. No surviving planktonic bacteria are recovered, although 104 biofilm bacteria survive this treatment. Design an experiment to identify genes important for biofilm resistance in P. aeruginosa. 

Biology, Academics

  • Category:- Biology
  • Reference No.:- M943153

Have any Question? 


Related Questions in Biology

Research paperguidelines for your research paper comparison

Research Paper Guidelines for Your Research Paper (Comparison / Contrast) 1. Title Page (begin on page1) 3 points A. Title is followed by Author's Name at the Top Portion. B. Is your title specific and informative? C. Do ...

1 explain the normal hormonal control of male sexual

1) Explain the normal hormonal control of male sexual development by the hypothalamus pituitary-gonadal axis. 2) Are there other hormones that might influence reproductive maturation? Which ones and how do they affect gr ...

What is the most common dna repair system in humans

What is the most common DNA repair system in humans, involving enzymatic functions and replacement of damaged DNA bases?

1 how cando you reduce the amount of waste and pollution

1. How can/do YOU reduce the amount of waste and pollution that you create?  In other words, describe 3 things that you can do/already do to reduce, reuse and recycle more. 2. The City of Phoenix recently started a progr ...

How does the presence of nitrate phosphate and ammonium

How does the presence of nitrate + phosphate and ammonium ions affect fertilization?

Healthy people 2020 is a framework to assist communities in

Healthy People 2020 is a framework to assist communities in preventing disease and promoting health. Search the scientific literature and find a research study related to one of the focus areas identified by Healthy Peop ...

Lets discuss the role of the intestinal microbiota in the

Let's discuss the role of the intestinal microbiota in the maintenance of health and the many opportunistic pathogens that cause disease when the delicate balance of normal microbiota in this region of the body is distur ...

What is the name of the class of enzymes that catalyze the

What is the name of the class of enzymes that catalyze the binding of specific amino acids to the proper tRNA? What would happen to the proteins in a cell if there was a mutation in the gene for that enzyme such that it ...

The mutation for huntingtons disease appears to have

The mutation for Huntington's disease appears to have originated in Western Europe, with a prevalence of 7 in 100,000 people. However, an isolated population in Lake Maracaibo in Venezuela, of European descent, have an i ...

What did the experiments by stanley miller demonstrate

What did the experiments by Stanley Miller demonstrate about the formation of the first organic molecules? Why was it significant?

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Section onea in an atwood machine suppose two objects of

SECTION ONE (a) In an Atwood Machine, suppose two objects of unequal mass are hung vertically over a frictionless

Part 1you work in hr for a company that operates a factory

Part 1: You work in HR for a company that operates a factory manufacturing fiberglass. There are several hundred empl

Details on advanced accounting paperthis paper is intended

DETAILS ON ADVANCED ACCOUNTING PAPER This paper is intended for students to apply the theoretical knowledge around ac

Create a provider database and related reports and queries

Create a provider database and related reports and queries to capture contact information for potential PC component pro

Describe what you learned about the impact of economic

Describe what you learned about the impact of economic, social, and demographic trends affecting the US labor environmen