Describe the mechanism and effects of DNA.
We will now deliberate the mechanisms as well as effects of DNA methylation on gene expression as well as discuss techniques for studying DNA methylation.
At the end of this section, you will be able to
•define patterns of DNA methylation throughout the genome
•name inhibitors of DNA methylation
•deliberate the impact of DNA methylation on gene transcription
•define techniques used to study DNA methylation
Select one of the approaches labelled by Dr. Esteller in the assigned journal article. First, provide an ephemeral description of the method in your own words then answer the following problems.
1. When or why would you use this method over other methods? Enquire yourself the following problems:
What are you looking for?
What are your starting materials?
Are there time, labour, and other cost considerations?
What do you expect to find?
2. What are the strengths and drawbacks of this method?