Question: DAALAQTAHRLKGVFAMLN after phosporylation of His by RcsC the following pKas are revelead: N terminus= 9.5 and C-terminus 4.5 Asp side chain: 3.3 ; Lys: 10.5 Arg side chain: 12.0 and His side chain is 6.0. Upon phosphorylation, the histidine imidazole pKA shifts to 6.5 and the phospho group has pKas of 2.0 and 6.8
- calculate the pI of each oligopeptide