Cross was made between two strains of plants that are agriculturally important, one strain was disease resistant but herbicide sensitive, the other strain was disease sensitive but herbicide resistant. A plant breeder crossed the two plants and then allowed the F1 generation to self-fertilization. The following data were obtained:

F1 generation: All offspring are disease sensitive and herbicide resistant
F2 generation: 157 disease sensitive, herbicide resistant
57 disease sensitive, herbicide sensitive
54 disease resistant, herbicide resistant
20 disease resistant, and herbicide sensitive

Perform a chi-square analysis to demonstrate if the inheritance pattern obeys the law of independent assortment. Please show the steps of calculation. The Chi-square table is attached.