1. Consider the three major classes of biologically important molecules: proteins, lipids, and nucleic acids. Which steps of the miniprep procedure act on proteins? on lipids? on nucleic acids?
2. Which aspect of plasmid DNA structure allows it to renature efficiently in step 8?
Step 8; Add 150 ?l of ice-cold KOAc solution to each tube. Close the caps, and mix the solutions by rapidly inverting the tubes about five times. A white precipitate will immediately appear.
3. What other kinds of molecules, in addition to plasmid DNA, would you expect to be present in the final miniprep sample?? How could you find out?