Consider the signaling pathway which proceeds via three protein kinases which are sequentially activated by phosphorylation. In one case the kinases are held in a signaling complex by a scaffolding protein; in the other, the kinases are freely diffusing. Describe the properties of these two kinds of organization in terms of signal amplification, speed and potential for cross-talk between the signaling pathways.
In words of signal amplification I believe the signaling complex by the scaffolding protein would be more proficient since it is organized. As well in terms of speed it is simpler to move if all kinases are ordered in an organized way.