Compare and contrast the prokaryotic replication fork to the eukaryotic one. Please discuss the differences in the polymerases involved, the difference in structure and mechanism of the leading and lagging strand synthesis, and the difference in the rolce of the sliding clamp.

Describe the initiation steps at the E. coli OriC. What would happen if the DnaA protein was mutated such that it could not bind ATP?

Describe how the Retinoblastoma (Rb) protein controls entry into S-phase. What would happen if Rb was mutated such that it could not be phosphorylated?

Describe how p53 controls cell cycle arrest after DNA damage.