Chromatographic assay reveals the total amount of T3 in the blood is 10 micrograms. You find that the various bound forms of the hormone are:
T3 (free)= 0.5%
T3 (albumin)= 12%
T3 (TBG)= 69%
T3 (pro-albumin)= 18.5%
Target tissue: heart whose Bmax of T3 receptors= 76 fmol of T3 receptor/mg protein ( 1 fmol=10^-15 mol)
where you have 100 micrograms protein in your heart assay. MW=68kDal
Calculate the amount (in grams) of available T3 and its receptor (also in g). How do the 2 amounts compare? Will Bmax be reached?