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Case History: Mapping a Bacterial Chromosome
Background
Conjunction mapping can be used to locate genes on a bacterial chromosome. In this ex, the F+ strain is able to synthesize methionine, valine, leucine, and histidine. The F- strain is unable to synthesize these amino acids; therefore, they must be supplied in the growth medium. Use the results from replica-plating to answer the problems.

problems
1. On which media, if any, should the F+ be able to grow? The F-?
2. Identify the prototrophs and auxotrophs. How can you tell?
3. Can you map the chromosome of this species? What further information do you need?

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Biology, Academics

Category:- Biology
Reference No.:- M9114003

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