Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Biology Expert

1. Electron transport translocates protons from the mitochondrialmatrix to the external medium, establishing a pH gradient across the inner membrane (outside more acidic than inside). The tendency of protons to diffuse back into the matrix is the driving force for ATP synthesis by ATP synthase. During oxidative phosphorylation by a suspension of mitochondria in a medium of pH 7.4, the pH of the matrix has been measured as 7.7.
a. find out [H+] in the external medium and in the matrix under these conditions.
b. What is the outside: inside ratio of [H+] ? Is there energy inherent in this concentration? describe.
c. find out the number of protons in a respiring liver mitochondrian, assuming its inner matrix compartment is a sphere of diameter of 1.5 micro meter.

2. find out:
a. The protonmotive force across the inner mitochondrial membrane at 25 degree celsius when the elcetrical difference is -0.18 V (inside negative), the pH outside is 6.7, and the pH inside is 7.5.
b. The percentage of energy from the chemical (pH) gradient and the percentage from the charge gradient.
c. The total free energy available for the phosphorylation of ADP.

3. In the presence of saturating amounts of oxaloacetate,the activity of citrate synthase from pig heart tissue shown a sigmoid dependence on the concentration of acetyl-CoA. When succinyl- CoA is added, the curve shifts to the right and the sigmoid dependence is more produced.
a. On that basis of those observations, suggest how succinyl- CoA regulates the activity of citrate synthase.
b. Why is succinyl - CoA an appropriate signal for the regulation of the citric acid cycle?
c. How does the regulation of citrate synthase control the rate of cellular respiration in pig heart tissue?
4. Electron transport translocates protons from the mitochondrialmatrix to the external medium, establishing a pH gradient across the inner membrane (outside more acidic than inside). The tendency of protons to diffuse back into the matrix is the driving force for ATP synthesis by ATP synthase. During oxidative phosphorylation by a suspension of mitochondria in a medium of pH 7.4, the pH of the matrix has been measured as 7.7.
a. find out [H+] in the external medium and in the matrix under these conditions.
b. What is the outside: inside ratio of [H+] ? Is there energy inherent in this concentration? describe.
c. find out the number of protons in a respiring liver mitochondrian, assuming its inner matrix compartment is a sphere of diameter of 1.5 micro meter.

4. find out:
a. The protonmotive force across the inner mitochondrial membrane at 25 degree celsius when the elcetrical difference is -0.18 V (inside negative), the pH outside is 6.7, and the pH inside is 7.5.
b. The percentage of energy from the chemical (pH) gradient and the percentage from the charge gradient.
c. The total free energy available for the phosphorylation of ADP.

5. In the presence of saturating amounts of oxaloacetate,the activity of citrate synthase from pig heart tissue shown a sigmoid dependence on the concentration of acetyl-CoA. When succinyl- CoA is added, the curve shifts to the right and the sigmoid dependence is more produced.
a. On that basis of those observations, suggest how succinyl- CoA regulates the activity of citrate synthase.
b. Why is succinyl - CoA an appropriate signal for the regulation of the citric acid cycle?
c. How does the regulation of citrate synthase control the rate of cellular respiration in pig heart tissue?

Biology, Academics

  • Category:- Biology
  • Reference No.:- M924115

Have any Question? 


Related Questions in Biology

There is a common misconception that traditional taxonomy

There is a common misconception that traditional taxonomy is based on morphology, while cladistics is based on genetic data. How is this misconception incorrect?

Freckles f and six fingers s are dominant alleles in humans

Freckles (F) and six fingers (S) are dominant alleles in humans and the genes occur on different chromosomes. If an FfSs male and an FfSs female have a WHOLE BUNCH of kids, Here are the expected ratios below. 9: freckles ...

What is the state of the issue of genome engineering

What is the state of the issue of Genome engineering technology in Sweden? Are there laws governing the it? Is there a high level of controversy in Sweden over the ethical issues of Genome engineering technology? Why/why ...

You have been in hr for 8 years three weeks ago you joined

You have been in HR for 8 years. Three weeks ago you joined your third employer, Chicken Pluckers, Inc., as HR Manager. Chicken Pluckers is a family-owned, meat processing plant with 400 employees. Your new boss, the CEO ...

You believe that the cytosolic protein p007 is involved in

You believe that the cytosolic protein p007 is involved in mediating growth hormone-stimulated proliferation in cells. You wish to establish a cell model using your cultured fibroblasts. Provide clear explanations to the ...

Embryonic pattern specification during development in some

Embryonic pattern specification during development in some cases requires maternally contributed ‘determinants', and in others is regulated by signaling between cells. For each of the following developmental events, indi ...

1 uterine contractions that are irregular and brief that

1) Uterine contractions that are irregular and brief that occur toward the end of gestation are called ________. 2) The hollow cavity within the blastocyst is the ________. 3) The process by which a viable embryo is form ...

Looking for assistance with the below information regarding

Looking for assistance with the below information regarding alternatives to landfills.  References are needed. Alternatives to Landfills The use of sanitary landfills for burial of municipal solid wastes has been the pri ...

1 e coli is a shortened name for a bacterium that lives in

1. E coli is a shortened name for a bacterium that lives in our gut. According to the rules of binomial nomenclature it is properly written as _______________. a. Escherichia COLI b. Escherichia Coli c. Escherichia coli ...

Lab reportthe purpose of a biology lab report is to

Lab Report The purpose of a Biology lab report is to describe and analyze a laboratory experiment that explores a scientific concept so that other scientists might be able to replicate the experiment and investigate the ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Section onea in an atwood machine suppose two objects of

SECTION ONE (a) In an Atwood Machine, suppose two objects of unequal mass are hung vertically over a frictionless

Part 1you work in hr for a company that operates a factory

Part 1: You work in HR for a company that operates a factory manufacturing fiberglass. There are several hundred empl

Details on advanced accounting paperthis paper is intended

DETAILS ON ADVANCED ACCOUNTING PAPER This paper is intended for students to apply the theoretical knowledge around ac

Create a provider database and related reports and queries

Create a provider database and related reports and queries to capture contact information for potential PC component pro

Describe what you learned about the impact of economic

Describe what you learned about the impact of economic, social, and demographic trends affecting the US labor environmen