bioinformatic You have been given a gene sequence, which is in RAW file format. Perform the following tasks by visually inspecting the sequence using information you were provided during lectures for Module 1, Topics 2 and 3. CCCTGGTTATTTATTGACAGGGGTATATAGTCCAGTGGTAGGCTGGATTA TATAATTTATTGAAATTTTCGATAAATTCCGTTATAATAATATTAAATCT GAAAATATTTAGTAAAAGGATGTGAGATATGTGAAGCTAAAACGCTTGTC TTTCTTCATGTTTGTTACCTTACTGGTATTTATATCTGTTTTTCCTGTTT ATGCAAACGATTTCGAAAAACATGGTACATATTATGAAATTTTTGTCAGG TCTTTTTATGACTCTGATGGTGACGGAATAGGGGATTTGAAAGGTATAAT AGAAAAACTGGATTATCTTAATGATGGAGACCCTGAAACCATTGCTGATC TGGGGGTTAATGGTATCTGGTTAATGCCTATCTTTAAATCTCCCTCCTAT CATGGCTATGATGTAACCGATTATTATAAGATTAATCCTGACTACGGGAC TCTGGAAGACTTCCATAAGCTTGTTGAGGCTGCCCATCAAAGGGGAATCA AGGTTATAATTGATTTACCCATCAATCATACCAGTGAAAGACATCCCTGG TTTCTCAAGGCTTCCCGGGATAAGAATAGTGAATACAGGGATTATTATGT CTGGGCTGGCCCCGATACCGATACCAAAGAAACCAAGTTAGATGGAGGCC GGGTCTGGCATTATTCCCCGACCGGCATGTATTATGGGTATTTCTGGAGT GGCATGCCTGATTTAAACTATAATAACCCTGAAGTTCAGGAAAAGGTTAT TGGGATAGCAAAATACTGGTTAAAACAGGGGGTTGATGGTTTCAGGCTTG ATGGAGCCATGCATATCTTCCCACCGGCCCAGTATGATAAAAACTTTACC TGGTGGGAGAAGTTCCGTCAGGAAATTGAAGAGGTAAAACCCGTTTACCT GGTGGGTGAGGTCTGGGATATTTCGGAAACGGTAGCTCCTTACTTCAAAT ATGGTTTTGATTCTACCTTTAACTTTAAACTGGCAGAGGCAGTTATCGCT ACGGCTAAAGCTGGATTTCCCTTTGGTTTTAATAAAAAGGCAAAACATAT TTACGGGGTATATGACAGGGAGGTTGGATTTGGGAATTATATCGATGCTC CCTTCCTGACCAACCATGATCAGAACCGGATTTTGGACCAGCTTGGGCAG GATCGTAATAAGGCCAGGGTTGCTGCCAGTATTTATTTGACCTTGCCTGG TAATCCCTTTATTTACTATGGTGAAGAAATCGGTATGAGGGGGCAGGGGC CCCATGAAGTTATCAGGGAGCCCTTCCAGTGGTATAATGGATCCGGGGAG GGAGAAACATACTGGGAGCCAGCCATGTATAATGATGGCTTTACTTCTGT TGAACAGGAAGAAAAGAATCTCGATTCCCTCTTAAATCACTACAGGAGGT TAATCCATTTCCGGAACGAAAATCCTGTCTTTTATACCGGTAAGATTGAG ATTATAAATGGAGGATTAAATGTAGTTGCATTTAGAAGATATAATGATAA GAGGGATTTATATGTCTACCATAACCTGGTAAACAGACCGGTTAAAATAA AAGTGGCTAGTGGTAACTGGACCTTATTGTTTAATTCAGGTGATAAGGAA ATTACCCCTGTTGAAGATAATAATAAACTTATGTATACTATCCCTGCTTA TACTACCATTGTTCTGGAAAAGGAGTAAAGGGAGAGGGTGAGTAATAATT ATGAAAAGGAGAGGGTAAATCCCTCTCCTTTTTTGTTAAA (a) Identify the possible Open Reading Frame (ORF). Hint: search for the START and STOP codons. (b) How many amino acids will the gene code for? (c) Translate the sequence and deduce the amino acid sequence for the putative protein. (d) Can you identify a signal peptide? If yes, identify it? (e) Sometimes, it is not possible to identify a gene using the method you used in question (a) above. One of the reasons is that the gene has not been sequenced completely - this happens during random sequencing of clones from bacterial genomic libraries. Perform a 6 frame translation of the given gene. Can you see the difference between the amino acid composition of all 6 translations. (f) Determine the G+C content of the coding region of the gene. Change the G+C content of the gene without changing the amino acid composition.