+1-415-315-9853

info@mywordsolution.com

 Algebra Math Calculus Physics Chemistry Biology Earth Science Physiology History Humanities English Sociology Nursing Science

1. Because G binds to C more strongly than A binds to T, the melting temperature should increase with the fraction of G/C pairs along the backbone of a sequence.

a. Plot the melting temperature as a function of G/C content for a 60 base pair sequence by varying the number of G/C pairs between 15 and 45 bases. Plot the melting temperature versus the fraction G/C content. Does the melting temperature depend linearly on the fraction of G/C pairs, and if so, what is the slope and the intercept? Make sure you show the sequences you used.

b. compare the melting temperature of the original strand (60 bases, 30 C/G bases) at different concentrations of salt, NaCl, up to physiological salt concentration (0.15 M NaCl).

2. To support 25 cycles of PCR in which you start with a single double strand where each strand is 4000 bases, what concentration of each nucleotide would you need to start with assuming each of the nucleotides is distributed throughout the DNA randomly? Assume 1 ml of solution is the total volume.

3. A 3700 bp sequence is digested with two separate restriction enzymes, KpnI and EcoR1. When digesting with KpnI, all EcoR1 sites are left in tact. Similarly, when digesting with EcoR1, all KpnI sites are left in tact. In the following, the results of two separate digestions are written. Assume each enzyme cuts with a staggered cut. What are the locations of the KpnI and EcoR1 sites on the 3700 bp original sequence?

KpnI digestion:
100 bp, no intact EcoR1 site, 5' staggered end, 3' staggered end.
500 bp sequence, with intact EcoR1 site 200bp from 5' end, 5' blunt end, 3' staggered end.
1400 bp sequence, 2 intact EcoR1 sites 200bp and 800 bp from 5' end, 5' staggered end, 3' staggered end.
500 bp, no intact EcoR1 site, 5' staggered end, 3' blunt end.
500 bp, intact EcoR1 site 300 bp from the 5' end, 5' staggered end, 3' staggered end.
700 bp sequence, no intact EcoR1 site, 5' staggered end, 3' staggered end.

EcoR1 digestion
600 bp sequence, no intact KpnI site, 5' staggered end, 3' staggered end.
1300 bp sequence, two intact KpnI sites, 300 bp and 1000 bp from the 5' end, 5' staggered end, 3' staggered end.
1100 bp, one intact KpnI site 600 bp from the 5' end, 5' staggered end, 3' blunt end.
200 bp, no intact KpnI site, 5' blunt end, 3' staggered end.
500 bp sequence, two intact KpnI sites, one 200 bp and one 300 bp from the 5' end, 5' staggered end, 3' staggered end.

• Category:- Biology
• Reference No.:- M924208

Have any Question?

## Related Questions in Biology

### Create a table to compare and contrast the chemical

Create a table to compare and contrast the chemical reactions catalyzed by the following enzymes as well as their mechanisms of action. Your answer should include at least 5 of the following where appropriate: the stereo ...

### 1 while working on snake blenny biochemistry you discover a

1. While working on snake blenny biochemistry, you discover a collection of five new molecules (a, b, c, d, e) shown below. The color of each molecule represents a distinct sugar. You decide to look for mutants to define ...

### Answer in a word or sentence1 what is decibel name any one

Answer in a word or sentence: 1. What is decibel? Name any one source that can produce noise of about 120 decibel. 2. What are the effects of smog on plants? 3. Define Pollutants with any one example. 4. What is acid rai ...

### Show work and explain answersq1in rats several

Show work and explain answers Q1: In rats, several independently assorting autosomal genes affect coat color. Gene A controls the distribution of yellow pigment in hair, and gene B causes black pigmentation. The two gene ...

You receive 12 samples in your laboratory for genetic screening. The samples form two families. The first family is composed of samples: 1(Mom Smith); 2 (Smith Son 1); 3(Smith Daughter 1); 4(Smith Son 2); 5(Smith Son 3); ...

### Physiology learning planfor your selected topic you are the

Physiology Learning Plan For your selected topic: You are the nephrology nursing educator working with new mill Topics -  GFR -  Removal of metabolic wastes -  Excretion of drugs and toxins -  Total body fluid volume and ...

### Which theoretical perspective that guides the nursing

Which theoretical perspective, that guides the nursing process with assessment of the family, do you find to be the most helpful and effective? Why is this theory more appealing to you than the others?

### Biological markers ndash nutritional status of an

Biological Markers – Nutritional Status of an individual Blood And The case of the mixed up case files Whats going on around here You have been handed a written report on an individual, but the labels on the biological s ...

### Assuming an od600 of 100 4 x 108 bacterial cellsml or the

Assuming an OD600 of 1.00 = ~ 4 x 108 bacterial cells/ml (OR the value provided by your TA), calculate the number of cells in your original undiluted bacterial suspension.

### Demonstrate your understanding of the phenotypic expression

Demonstrate your understanding of the phenotypic expression of genes based on modes of inheritance. Explain the evolutionary significance of different modes of inheritance; for example an autosomal dominant allele which ...

• 13,132 Experts

## Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

### A cola-dispensing machine is set to dispense 9 ounces of

A cola-dispensing machine is set to dispense 9 ounces of cola per cup, with a standard deviation of 1.0 ounce. The manuf

### What is marketingbullwhat is marketing think back to your

What is Marketing? • "What is marketing"? Think back to your impressions before you started this class versus how you

### Question -your client david smith runs a small it

QUESTION - Your client, David Smith runs a small IT consulting business specialising in computer software and techno

### Inspection of a random sample of 22 aircraft showed that 15

Inspection of a random sample of 22 aircraft showed that 15 needed repairs to fix a wiring problem that might compromise

### Effective hrmquestionhow can an effective hrm system help

Effective HRM Question How can an effective HRM system help facilitate the achievement of an organization's strate