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Basic Safety (Introduction)

1. At a minimum, this type of laboratory facility is necessary to handle organisms that typically do not cause disease in healthy individuals and present a minimal threat to the environment and lab personnel:

A. BSL-1

B. BSL-2

C. BSL-3

D. BSL-4

E. All of the above

2. At a minimum, this type of laboratory facility is necessary to handle microbes in the open without the need for special containment equipment:

A. BSL-1

B. BSL-2

C. BSL-3

D. BSL-4

E. All of the above

3. At a minimum, organisms commonly encountered in the community which present a moderate environmental and/or health hazard are handled in this type of laboratory facility:

A. BSL-1

B. BSL-2

C. BSL-3

D. BSL-4

E. All of the above

4. At a minimum, this type of laboratory facility is necessary to handle organisms associated with human diseases of varying severity:

A. BSL-1

B. BSL-2

C. BSL-3

D. BSL-4

E. All of the above

5. At a minimum, a Class I or Class II biosafety cabinet is required for work in this type of laboratory facility:

A. BSL-1

B. BSL-2

C. BSL-3

D. BSL-4

E. B and C

6. Organisms handed in this type of facility are associated with respiratory transmission and lethal diseases and this type of facility prevents infectious material from being aerosolized:

A. BSL-1

B. BSL-2

C. BSL-3

D. BSL-4

E. All of the above

7. Ebola virus, Marburg virus, Lassa fever and other agents causing hemorrhagic fever are handled in this type of laboratory facility:

A. BSL-1

B. BSL-2

C. BSL-3

D. BSL-4

E. All of the above

8. PPE includes the following:

A. Gloves

B. Lab coat

C. Face protection

D. Full body, air supplied positive pressure suit

E. All of the above

9. A Class II or Class III biosafety cabinet is required for work in this type of laboratory facility:

A. BSL-1

B. BSL-2

C. BSL-3

D. BSL-4

10. Our microbiology laboratory at Miami Dade College in room G316 is this type of laboratory facility:

A. BSL-1

B. BSL-2

C. BSL-3

D. BSL-4

11. Do not do the following in the microbiology laboratory:

A. Apply cosmetics

B. Handle contacts

C. Touch personal belongings with gloved hands

D. Eat and drink

E. All of the above

12. Do not do the following in the microbiology laboratory:

A. Smoke

B. Wear long jewelry

C. Wear long hair down

D. Wear open toed shoes

E. All of the above

13. It is necessary to turn off the Bunsen burner when it is not in immediate use

A. True

B. False

14. It is necessary to wear eye protection even if you wear glasses or contacts

A. True

B. False

15. Where do we dispose of microscope slides and cover slips?

A. Sharps container

B. Biohazard bag

C. Biohazard box

D. Regular trash

E. All of the above

16. Where do we dispose of glass tubes and petri dishes?

A. Sharps container

B. Biohazard bag

C. Biohazard box

D. Regular trash

E. All of the above

17. Where do we dispose of used gloves and paper towels?

A. Sharps container

B. Biohazard bag

C. Biohazard box

D. Regular trash

E. All of the above

18. What are students allowed to dispose of in the biohazard box?

A. Nothing

B. Trash

C. Bacteria

D. Only Dr. Madison can use biohazard box

E. A and D

19. Where do we dispose of used tube caps

A. Sharps container

B. Biohazard bag

C. They are recycled

D. They do not get disposed of

E. C and D

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