Ask Question, Ask an Expert


Ask Biology Expert

Based on the following information how do I solve for 10x buffer, uls HOH, ul Enzyme, and Total?

Setup a digest that contains 10 ug of DNA/Sample, 3.5 ug/ul stock DNA (from experiment with 5ul of stock diluted with 995 H2O), 40 units of HAE III enzyme, and enzyme stock of 12 units/ul.

Sample ul DNA uls 10xBuffer uls HOH uls Enzyme Total ul
1 10ug/3.5 ug/ul

Please confirm DNA column, and demonstrate or provide formulas for the remaining columns.

Biology, Academics

  • Category:- Biology
  • Reference No.:- M924987

Have any Question? 

Related Questions in Biology

Depletion of nadph and glutathione in cells is considered

Depletion of NADPH and glutathione in cells is considered harmful. Explain why, and give an example of a chemical that may deplete NADPH and glutathione through interaction with the FMO enzyme. The FMO3 enzyme has an imp ...

Trypanosoma brucei is the agent that causes african

Trypanosoma brucei is the agent that causes African sleeping sickness in humans bitten by the tsetse fly. Glycolysis is unusual in this organism, providing an opportunity to create drugs that poison the parasite without ...

Evolutionary biology in the age of genomics- q1

Evolutionary Biology in the age of Genomics- Q1. Phylogenetic trees- You are analyzing sequences sampled from four turtle species (A-D), considering a genomic region that is 75 base pairs long. You observe the following ...

Researchers are working to develop biofuels to free us from

Researchers are working to develop biofuels to free us from dependence on fossil fuels. Based on what you know about cellulose, what do you predict are the major advantage and disadvantage of using cellulose-rich plant m ...

Assignmentappendix c organizational requirements for

Assignment Appendix C: Organizational Requirements for Phototropism Virtual Laboratory Paper Introduction: Provide readers with the experiment's background information, and present the hypothesis in approximately 175 wor ...

Internet postings about whole foods and wild oatsfrom its

Internet Postings about Whole Foods and Wild Oats From its beginnings as one small store in Austin, Texas, Whole Foods Market has grown into the world's leading retailer of natural and organic foods, with hundreds of loc ...

Which theoretical perspective that guides the nursing

Which theoretical perspective, that guides the nursing process with assessment of the family, do you find to be the most helpful and effective? Why is this theory more appealing to you than the others?

Recall that the biological species concept defines a

Recall that the biological species concept defines a species as a group of populations whose members have the potential to interbreed in nature and produce viable, fertile offspring. This means that all members of these ...

Biological markers ndash nutritional status of an

Biological Markers – Nutritional Status of an individual Blood And The case of the mixed up case files Whats going on around here You have been handed a written report on an individual, but the labels on the biological s ...

Genetics and reproductioncreate at least a 350-word blog

Genetics and Reproduction Create at least a 350-word blog post in Microsoft® Word in response to the following question: Female copperhead snakes have the ability to reproduce both sexually and asexually. In your opinion ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

A cola-dispensing machine is set to dispense 9 ounces of

A cola-dispensing machine is set to dispense 9 ounces of cola per cup, with a standard deviation of 1.0 ounce. The manuf

What is marketingbullwhat is marketing think back to your

What is Marketing? • "What is marketing"? Think back to your impressions before you started this class versus how you

Question -your client david smith runs a small it

QUESTION - Your client, David Smith runs a small IT consulting business specialising in computer software and techno

Inspection of a random sample of 22 aircraft showed that 15

Inspection of a random sample of 22 aircraft showed that 15 needed repairs to fix a wiring problem that might compromise

Effective hrmquestionhow can an effective hrm system help

Effective HRM Question How can an effective HRM system help facilitate the achievement of an organization's strate