Ask Question, Ask an Expert


Ask Biology Expert



Baculovirus is widely used for expressing recombinant proteins. Structural of baculovirus is a rod-shaped nucleocapsid containing genomic DNA. The baculovirus Autographa California multiple nuclear polyhedrosis virus (AcMNPV) used as a vector in many gene therapies studies in the science world. The wild-type AcMNPV does not replicate in mammalian cells but it can transfer then. Due to higher level of gene expression, the baculovirus are active. In insect cell culture it easy to maintain the culture compared to mammalian cells. The infection of Baculovirus can turn off host cell protein expression and impair secretion apparatus of Endoplasmic reticulum (ER)-Golgi pathway proteins. But some strategies have been developed to improve secretion protein. Chaperones proteins are potential on making secrete protein production up to two fold. Secretory alkaline phosphate-EGFP fusion protein (SEFP) as co-expressed with chaperones, calreticulin (CALR) which located in storage compartment associated with ER. Alpha Synuclein (α-syn) and Beta Synuclein (β-syn) is a small protein, α-syn work as a small heat shock protein. Both of them are molecular chaperones.

The aim of this project is to enhance the expression of the SEFP testing chaperones (α-syn, β-syn with Calreticulin as a positive control, and dsRed as a negative control) which may increase and enabled correct folding protein forms. We will test promoters on virus expression levels: gp64 to use if timing of promoter expression/protein production is crucial to expression levels. -------- > more explanation about gp64! How it works?

In order for making Baculovirus based on AcMNPV this project will use Flash BacUltra. It is a new platform technology for the production of recombinant baculoviruses, optimised for producing 100 % recombinant virus by eliminating the need for plaque purification and it also increases protein yield.

The main content:

Project design:

Firstly, used the plaque assay (describe 4 viruses) to titre viruses (pfu/ml Multiplicity of Infection) and used the alkaline phosphatase activity assay to compare secreted protein in the 4 viruses. After comparing the viruses, continue with the western blot and SDS-PAGE to analyse(marker protein production using anti-GFP antibody), at the end observe the expression in fluorescence microscopy.


The expectation is to see the co-expressed SEFP and chaperones either α-syn or β-syn can deliver enhanced on secretion protein production in BEVS which can improve up to 2.3 fold.  

Design Project Proposal:

For this piece of coursework you will prepare a project proposal based on the actual project.

Your full project proposal should be between 2000 and 2500 words and should include:

• a scientific abstract (200 words),

• an abstract for a general audience (200 words),

• an introduction (including aim and objectives) up to 1000 words,

• materials and methods, including a description of the techniques you will use, how your data will be presented and analysed (including appropriate statistical tests),

• references (using Endnote to cite and list references in the bibliography in the Harvard style).

Biology, Academics

  • Category:- Biology
  • Reference No.:- M9515

Have any Question? 

Related Questions in Biology

In the following experiment students plated e coli top

In the following experiment, students plated E. coli (top right), S. epidermidis (top left), P. Aeruginosa (bottom right) and E. aerogenes (bottom left) on Trypticase soy agar (Yellow media), Glucose mineral salts agar ( ...

Show where cell types found in mature plants are located in

Show where cell types found in mature plants are located in a young growing root, for this example use corn. For this, draw a longitudinal section of the corn root to show these parts. Then describe the cell type and ind ...

Assignmentmutations occur when dna is not replicated

Assignment Mutations occur when DNA is not replicated properly. Fortunately, for the most part, cells have a mechanism to correct the mutation or destroy it. Sometimes that mechanism fails and the mutation survives. Spen ...

For the optimal temperature experiment which

For the optimal temperature experiment, which enzyme/substrate pair did you use? List your results for the absorbance at one minute when the temperature was 10, 20, 30, 40, 50, 60, 70 and 80 degrees. Based on your result ...

Human biologydiscussion 1- identify and compare the two

Human Biology Discussion 1- Identify and compare the two major cardiovascular circuits in the human body. Include the purpose of each and the organs involved. Do not cut and paste information from the text or other sourc ...

Human biology1where does lymph come from2list the primary

Human Biology 1. Where does lymph come from? 2. List the primary lymphatic organs. List the secondary lymphatic organs. 3. What structures allow the lymph to enter the cardiovascular system? 4. Why is the thymus so criti ...

Physiology learning planfor your selected topic you are the

Physiology Learning Plan For your selected topic: You are the nephrology nursing educator working with new mill Topics -  GFR -  Removal of metabolic wastes -  Excretion of drugs and toxins -  Total body fluid volume and ...

Assignment- applicationyou are responsible for creating

Assignment- Application You are responsible for creating your own answers. I have given you the information you need in previous lectures (from what I wrote on the board) for you to answer the questions. Also, some of th ...

In this assignment you will be writing a 1000-1250-word

In this assignment, you will be writing a 1,000-1,250-word essay describing the differing approaches of nursing leaders and managers to issues in practice. To complete this assignment, do the following: nursing shortage ...

The scientific language of god assignmentthree parts if

The Scientific Language of God Assignment Three parts if this assignment to submit on Blackboard 1. Write a 3-4 sentence executive summary for each of the following three article links- (That means a paragraph on each of ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

A cola-dispensing machine is set to dispense 9 ounces of

A cola-dispensing machine is set to dispense 9 ounces of cola per cup, with a standard deviation of 1.0 ounce. The manuf

What is marketingbullwhat is marketing think back to your

What is Marketing? • "What is marketing"? Think back to your impressions before you started this class versus how you

Question -your client david smith runs a small it

QUESTION - Your client, David Smith runs a small IT consulting business specialising in computer software and techno

Inspection of a random sample of 22 aircraft showed that 15

Inspection of a random sample of 22 aircraft showed that 15 needed repairs to fix a wiring problem that might compromise

Effective hrmquestionhow can an effective hrm system help

Effective HRM Question How can an effective HRM system help facilitate the achievement of an organization's strate