Question: Assume you isolate a mutant e.coli strain that is unable to grow without tryptophan supplementation at 37 degrees C but grows normally at 25 degrees C. what basic class of mutant strain is this?
which type of mutation is more likely to yield this phenotype?
a. misense mutation in an enzyme hydrophobic pocket
b. nonsense mutation in an enzyme active site
c. frameshift mutation early in the ORF
Why?
Question: Which one inhibits protein synthesis by irreveribly binding to the prokaryotic ribosome, and which one deactivates enzymes (two answers)?