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Question: Assume you have 2 tubes containing cultures of genetically distinct bacterial strains. You know that when you mix the two together, you get Horizontal gene transfer (HGT), but you do not know what genetic exchange mechanism is accomplishing this transfer. Prior to mixing the strains, you add an enzyme that degrades any DNA it comes into contact with the tubes and allow it to incubate briefly. You then mix contents of both tubes together and find that you are no longer able to achieve HGT. Both cultures are still completely viable. Name the 3 methods of HGT that we discussed in class. Which of these is the MOST likely process you interfered with? In your answer, explain your observations and why the other two processes are not likely to be involved.

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