Question: Assume you are studying a microorganism from a hot spring that uses organic carbon as an energy and carbon source. The microorganism is a facultative anaerobe and can use nitrate as a terminal electron acceptor. Your lab partner inadvertently mixes your isolate with a number of other microorganisms in the lab. To continue your research, you need to re-isolate the hot spring microbe.
a) Develop an enrichment medium and conditions to support the growth of your microbe.
b) Discuss the steps you would take to recover your original microorganism.