1. If you take a mature prokaryotic mRNA, and use reverse transcription to create DNA from it, will it resemble the original DNA sequence??
Answer
A Yes, it will look like the original DNA sequence
B No, it may be missing one or more exons
C No, it will be missing all the introns
D No, it will be missing introns and potentially one or more exons

2. In the example of the gene that encodes antibodies in humans, alternative splicing produces:
Answer

A. the same protein from two different genes, one with a transmembrane domain and one without

B. two mature (processed) mRNAs from the same gene, one with a transmembrane domain and one without
C. two proteins from the same mature (processed) mRNA, one with the transmembrane domain excised during post-translational processing

D. Two proteins from the same processed (mature) mRNA, with the transmembrane portion of one spliced out after translation

3. Can transcription and translation ever occur simultaneously?
Answer

A. No, because ribosomes cannot translate unprocessed mRNA

B. No, because of the nuclear membrane separating DNA from the ribosomes that do the translation

C. No, because the processes are so different

D. Yes, in prokaryotes

4. The codon "ATG" encodes methionine. What is the correct anticodon sequence in the gene that codes for tRNA (thus written 5' to 3')?
Answer

A. 5' AUG 3'

B. 5' UAC 3'

C. 5' CAU 3'

D. 5' GUA 3'

5. How does a ribosome know where on an mRNA to start translating?
Answer

A. It picks any AUG

B. It picks the first AUG on the mRNA

C. It picks the first AUG following a particular consensus sequence

D. It begins with the first codon following the 5' cap