Assignment

Introduction:

For this task, complete the Antibiotic Sensitivity-Kirby-Bauer Diffusion Test experiment. The instructions for completing the experiment are found at the "Antibiotic Sensitivity-Kirby-Bauer Diffusion Test" web link below. Then, you will write a laboratory report discussing the results of this experiment.

Your lab report should be written in complete sentences and in paragraph form. Your report should include the following sections:

• introduction
• materials and methods
• results
• discussion

The information required for each section is outlined in the requirements section below.

Requirements:

Your submission must be your original work. No more than a combined total of 30% of the submission and no more than a 10% match to any one individual source can be directly quoted or closely paraphrased from sources, even if cited correctly. Use the Turnitin Originality Report available in Taskstream as a guide for this measure of originality.

You must use the rubric to direct the creation of your submission because it provides detailed criteria that will be used to evaluate your work. Each requirement below may be evaluated by more than one rubric aspect. The rubric aspect titles may contain hyperlinks to relevant portions of the course.

A. Write a lab report about the Antibiotic Sensitivity-Kirby-Bauer Diffusion Test experiment in which you do the following:

1. Explain the relationship between antibiotics and selective toxicity.

a. Discuss the relative benefits of broad-spectrum and narrow-spectrum antibiotics.

Note: The explanation from part A1 and discussion from part A1a belong in the introduction section of the lab report.

2. Describe the steps you followed to complete the Kirby-Bauer diffusion test.

Note: Include any changes from the normal protocol that might have been incorporated into your experiment.

Note: The description from part A2 belongs in the materials and methods section of the lab report.

3. Record the observed results of the Kirby-Bauer diffusion test, including the zone of inhibition measurement and interpretation (e.g., resistant, intermediate, susceptible) for each antibiotic using the attached "Data Table."

Note: The explanation from part A3 belongs in the results section of the lab report.

4. Based on your recorded results from the Kirby-Bauer diffusion test, explain whether the following antibiotics are effective in controlling S. epidermidis:

• penicillin
• novobiocin
• gentamicin

Note: The explanation from part A4 belongs in the discussion section of the lab report.

B. Acknowledge sources, using in-text citations and references, for content that is quoted, paraphrased, or summarized.

C. Demonstrate professional communication in the content and presentation of your submission.