As you continue on in your career, you become keenly interested in the mechanisms of immune cell recruitment during the innate immune response. After years of graduate work, you discovered a new cytokine (X), which chemoattracts neutrophils. In your excitement you begin devising experiments to characterize the function and relevance of cytokine X.
- From what you know of chemokines, predict the general structure of chemokine X and its cognate receptor.
- Where, by what cell types, and when would you expect it to be expressed? Explain, experimentally, how you would test your hypothesis.
- What is the effect of expression of chemokine X on the immune response. Explain, experimentally, how you would test your hypothesis.
As you are working in the lab, you obtain a major career-defining result. You have discovered a new TLR (TLR15), which recognizes either a bacterial or viral antigen. Your task to finish graduate school is to fully characterize the molecule.
- Design an experiment to determine if your new TLR15 recognizes a bacteria or a virus. What results do you anticipate and what experimental difficulties will you potentially encounter?
- Comment on the likely structure for the molecule and indicate a plausible signaling pathway for cellular activation.
- Where in the cell would you expect to find this new TLR? Why? Please design and fully explain two different experiments to test your hypothesis.
- What are the consequences of deficiency of TLR15? Please design and fully explain two different experiments to test your hypothesis.