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Answer the following questions.

1) When extracting DNA why should you use an enzyme?

a. Unwind from packing protein

b. Pull cells apart

c. Slow down extraction process

d. Protect DNA from degrading

2) Which of the following protects the ends of the DNA molecule?

a. Detergent

b. Salt

c. Water

d. Alcohol

3) What do you use to separate the cells from each other?

a. Strainer

b. Blender

c. ROH

d. Toothpick

4) Why does DNA end up in the alcohol layer at the end of the procedure?

a. DNA sinks because it's more dense than the banana mixture

b. DNA sinks because it's less dense than the banana mixture

c. DNA floats because it's denser than the banana mixture

d. DNA floats because it's less dense than the banana mixture

5) Why do you add detergent to the banana scraps?

a. Pull the cells apart from each other

b. Protect the DNA

c. Dissolve the cell and nuclear membrane

d. Change the density of the DNA

6) What are two things or conditions that could damage DNA if it is not protected by salt in Step 1 of the procedure

7) What would happen if you forgot to add the meat tenderizer? Explain why.

8) What are at least two uses of the extracted DNA other than for forensics?

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