Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Biology Expert

A standard procedure in pufying tubulin is to extract the tissue on ice, then add GTP and warm the tissue extract to 37C. The warmed extract is then centrifuged at a speed insufficient to pellet average sized proteins, but sufficient to pellet large protein complexes. After centrifugation, the supernant is discarded, and the solution is agian warmed for a few minutes. Then centrifugation at the same speed described above is again performed. This process is repeated several times.

How would this processs result in purification of tubulin?

What contaminating proteins would likely co-purify with the tubulin?

Biology, Academics

  • Category:- Biology
  • Reference No.:- M925280

Have any Question? 


Related Questions in Biology

Cell cycle checkpoints are an important feature of a

Cell cycle checkpoints are an important feature of a dividing cell to reduce errors that can occur in various stages of the cell cycle. Loss of Rb, an important part of the G1-S transition checkpoint, can result in uncon ...

1 describe the three classes of carcinogens and their sub

1. Describe the three classes of carcinogens and their sub classes; describe the role each plays in the cancer process and give chemical examples. 2. Describe six points in the cancer process where the process can be inh ...

The sheep dolly was the first animal cloned by

The sheep Dolly was the first animal cloned by transplanting the nucleus from a cell from an adult sheep into an egg of a donor sheep. Dolly appeared to grow normally for several years, but at age 6, she developed compli ...

Anesthesiathere are two questions to this discussion

Anesthesia There are two questions to this discussion; answer  both  questions. Question A The textbook says that the common anesthesia time units are 15 minutes, but that some carriers use 30 minutes. Not mentioned in t ...

What do we call the transfer of pollen grains from anther

What do we call the transfer of pollen grains from anther to the stigma in plants?

What would be the expected genotypes and phenotypes of the

What would be the expected genotypes and phenotypes of the children and their relative frequencies from the following crosses a) NN X nn b) Nn x nn c) Nn x Nn d) nn x nn Where n represent gene for albinism and N represen ...

In high altitudes is it necessary to have more or less

In high altitudes is it necessary to have more or less hemoglobin? Why is carbon monoxide toxic for humans? How is heart contraction triggered?

Question 1future drugs and vaccines for the use against

Question 1 Future drugs and vaccines for the use against Ebola Pick an experimental drug, vaccine or other treatment that is being considered in the fight against Ebola. http://www.cdc.gov/vhf/ebola/outbreaks/2014-west-a ...

Antimicrobial resistance and natural selectionthe use of

Antimicrobial Resistance and Natural Selection: The use of antimicrobial drugs began during the World War II era. After more than 50 years of widespread use, many antimicrobial drugs have become ineffective. Health autho ...

How to apply kantian and utilitarian theories to the

How to apply Kantian and Utilitarian theories to the situation that existed at Memorial Medical Center in the days immediately after Hurricane Katrina, was Dr. Anna Pou's treatment of the LifeCare patients on the seventh ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Section onea in an atwood machine suppose two objects of

SECTION ONE (a) In an Atwood Machine, suppose two objects of unequal mass are hung vertically over a frictionless

Part 1you work in hr for a company that operates a factory

Part 1: You work in HR for a company that operates a factory manufacturing fiberglass. There are several hundred empl

Details on advanced accounting paperthis paper is intended

DETAILS ON ADVANCED ACCOUNTING PAPER This paper is intended for students to apply the theoretical knowledge around ac

Create a provider database and related reports and queries

Create a provider database and related reports and queries to capture contact information for potential PC component pro

Describe what you learned about the impact of economic

Describe what you learned about the impact of economic, social, and demographic trends affecting the US labor environmen