1. Insertion sequences target which areas on a target DNA sequence?
A. A sequence of nucleotides identical to the inverted repeat sequence found on the insertion sequence itself
B. The gene that codes for transposase
C. A unique inverted repeat sequence not found on the insertion sequence
D. A gene coding for antibiotic resistance

2. What makes an insertion sequence different from other DNA sequences found in a cell?
A. They can integrate into the cell genome.
B. They contain inverted repeats.
C. They are capable of effecting their own movement from one location to another on DNA.

3. If the gene that codes for transposase is mutated so that it no longer produces the fully functional enzyme, how will the insertion sequence be affected?
A. The transposase enzyme would cut randomly in the host genome, effectively killing the cell.
B. The insertion sequence would lose its ability to effect its own movement.
C. Nothing; the transposase gene has no known role for mobilizing insertion sequences.
D. The insertion sequence would randomly insert itself into new locations in the DNA.

4. Which of the following might result in a frameshift mutation?
A. Nitrous acid
B. 5-bromouracil
C. Benzopyrene

5. A new chemotherapeutic agent is developed that alters the structure of all thymines in DNA. These thymines are then misread during the production of mRNA. Which of the following could NOT result from this type of mutation?
A. silent mutation
B. missense mutation
C. nonsense mutation
D. frameshift mutation

6. While studying protein synthesis in the lab, you experimentally induce a mutation in a bacterium's DNA. You assess the proteins in the mutated bacterium and find that one of them is significantly shorter than its wild-type counterpart. Based on this information, what type of mutation did you induce?
A. deletion mutation
B. missense mutation
C. nonsense mutation
D. silent mutation

7. Why does conjugation between an Hfr strain and an F- strain not result in two Hfr strains?
A. The cell membranes between the two strands never fuse together.
B. Conjugation is typically disrupted before the fertility factor can be transferred.
C. The transferred genes typically recombine with the recipient chromosome.
D. Hfr strains lack fertility factor.

8. Which of the following is a characteristic of an F+ cell?
A. Ability to synthesize sex pili
B. Presence of a fertility factor
C. Ability to synthesize sex pili, presence of a fertility factor, and ability to mate with an F- cell.
D. Ability to mate with an F- cell

9. What benefit does the F- strain receive from mating with an Hfr strain?
A. It becomes an F+ cell.
B. It acquires new, potentially beneficial genes from the Hfr strain.
C. It picks up a fertility factor.
D. It can now produce sex pili.