A generalized transducing phage is used to transduce an a-b- c- d- e- recipient strain of E. coli with an a+ b+ c+ d+ e+ donor. The recipient culture is plated on various media with the results shown in the following table. (Note that a- indicates a requirement for A as a nutrient, and so forth.) What can you conclude about the linkage and order of the genes?
Compounds added Presence (+) or to minimal medium absence (-) of colonies
CDE -
BDE -
BCE +
BCD +
ADE -
ACE -
ACD -
ABE -
ABD +
ABC -