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A 40-year-old male who was seropositive for HIV experienced abdominal pain, fatigue, and low-grade fever (38°C) for 2 weeks. A chest X-ray examination revealed lung infiltrates. Gram and acid-fast stains were negative. A viral culture revealed the cause of his symptoms: a large, enveloped polyhedral virus with double-stranded DNA. What is the disease; Which virus caused it; Why was the viral culture done after the Gram and acid-fast stain results were obtained.

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