Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Biology Expert

A 2500 base-pair long cDNA of unknown sequence was cloned to the MCS of a plasmid vector which has universal priming sequences adjacent to and on both sides of the MCS. On one side this is termed as the "forward universal priming sequence" and on the other it is termed as the "reverse universal priming sequence." Sanger sequencing was done by using primers which are specific for each of these universal priming sequences and around 700 nucleotides of sequence was determined from each primer. Supposing it is not possible to get sequence analyses longer than 700 nucleotides, describe the steps which should be done to obtain the remainder of the sequence of the cloned cDNA.

Hint: There are two very different methods which will accomplish this. One method needs much more effort and is less reliable than the other. Either method will receive full credit.

Biology, Academics

  • Category:- Biology
  • Reference No.:- M937116

Have any Question? 


Related Questions in Biology

Please read instructions carefully 4-5 body page paper must

PLEASE READ INSTRUCTIONS CAREFULLY: 4-5 body page paper, must include a title and reference page, CITE PROPERLY,  I run all papers through turnitin website before i accept them which means you paper can only have 10-15% ...

What is the difference between prokaryotes and

What is the difference between prokaryotes and eukaryotes?

What is the difference between the brain and cerebrumwhat

What is the difference between the brain and cerebrum? What are the main parts of these structures? How is the cerebrum anatomically divided? How is it structurally explained that the motor activity of the left side of t ...

Explain in detail the physiological cause of huntington

Explain, in detail, the physiological cause of Huntington Disease. Describe its symptoms and the physiological cause for each, and describe all available treatments and the physiological changes that each treatment cause ...

Compare the path of an amino acid to that of a large fatty

Compare the path of an amino acid to that of a large fatty acid from absorption to delivery to a cell.

Question 1briefly explain the control of feeding by the

Question 1 Briefly explain the control of feeding by the hypothalamus. Question 2 Compare and contrast the functions of estrogen, testosterone and oxytocin in reproductive behavior. Question 3 What evidence shows that th ...

Write an essay on the differences and similarities of

Write an essay on the differences and similarities of creation science and intelligent design creationism, and how do they stand up as actual science?

1 how is gene expression regulated give three different

1. How is gene expression regulated (give three different forms of regulation mechanisms)? 2. How does gene regulation relate to cancer development?

Identifynbspan organism that lives within 50 miles of your

Identify  an organism that lives within 50 miles of your home.  I live in Panama City, Florida 32405 I would like to use the wolf please? Write  a 1,050- to 1,400-word paper about how the organism has adapted to survive ...

A 74-year-old woman is transported to a regional burn

A 74-year-old woman is transported to a regional burn center after being rescued from a house fire. Apparently, a space heater was knocked over and an area rug caught fire. The fire quickly spread throughout the house. S ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Section onea in an atwood machine suppose two objects of

SECTION ONE (a) In an Atwood Machine, suppose two objects of unequal mass are hung vertically over a frictionless

Part 1you work in hr for a company that operates a factory

Part 1: You work in HR for a company that operates a factory manufacturing fiberglass. There are several hundred empl

Details on advanced accounting paperthis paper is intended

DETAILS ON ADVANCED ACCOUNTING PAPER This paper is intended for students to apply the theoretical knowledge around ac

Create a provider database and related reports and queries

Create a provider database and related reports and queries to capture contact information for potential PC component pro

Describe what you learned about the impact of economic

Describe what you learned about the impact of economic, social, and demographic trends affecting the US labor environmen