A 1 kb region is amplified by pcr for four cycles. what, Ask an Expert

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The genome of virus SV40 is a circular double-stranded DNA molecule 5 kb long.

a. A 1 kb region is amplified by PCR for four cycles. What fraction of the total DNA does the target sequence constitute? Show your work.

b. After many generations of growth in medium containing 14N, the medium was replaced with new medium containing 15N. After five generations, what is the ratio of light:heavy DNA strands? Show your work.

c. If the number of thymine in the SV40 genome is 1830, what is the percentage of cytosine in the genome? Show your work.

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Category:- Biology
Reference No.:- M9399804

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