(Answered) 1if you spread 01 ml of 10-3 of a sewage sample on a plate

Academics

1. If you spread 0.1 ml of 10-3 of a sewage sample on a plate, and obtained 221 colonies on the plate after incubation, how many CFU/ml were present in the undiluted sewage sample?

2. How can you make E.coli cells competent so that they can be transformed?

3. What is the appearance of transformed bacterial colonies?

4. After transformation procedure, your test tube may contain both transformed and nontransformed cells.
a. which will grow on LB plate? Which will grow on LB/amp plates?
(+ DNA or - DNA)

5 Why is there heavy growth on LB and very little growth on LB/amp?

5. What is the reason to plate E.coli cells on LB/amp?

6. Why are there both green and white colonies on LB/amp?

View complete question

Biology, Academics

Category:- Biology
Reference No.:- M91606652
Price:- $30

Verified Expert

Priced at $60 Now at $30, Verified Solution

Have any Question?Write your Review or question?

Related Questions in Biology

If you diluted 1 ml of a sample into 9 ml of water then

If you diluted 1 ml of a sample into 9 ml of water, then took 1 ml of that and diluted into another 9 ml of water, and did that another two times, what is fold the dilution factor?

Bioinformatics assignment -in this assignment should check

Bioinformatics Assignment - In this assignment should check the following sequence and test whether it has the following restriction cut sites. This searching should be done globally, that is, it should check for all pos ...

How do i calculate the number of molecules needed for

How do I calculate the number of molecules needed for lethal dose? I am given the chemical name's LD50 and the "Estimated Lethal Dose (in grams) for 60kg human.

I am having trouble identifying if my buffer solution is a

I am having trouble identifying if my buffer solution is a weak acid and salt....or weak base and salt. Often my questions just give me then names of two molecules and I do not understand how to differentiate. For exampl ...

Question in portugal employers are not allowed to terminate

Question: In Portugal, employers are not allowed to terminate employees. In Japan, employers are required to measure the waistlines of their employees, and anyone whose waist exceeds the allowed size is put on a diet by ...

Experiment 1 symmetry in common objectsreview the objects

Experiment 1: Symmetry in Common Objects Review the objects listed below (many of which can be found in your lab kit). Decide what type of symmetry they possess. Explain why you chose the type of symmetry you did. 1. Saf ...

Is the iso-osmotic point different for different solutes

Is the iso-osmotic point different for different solutes? Please specify in what ways can the iso-osmotic points be different and why there is a difference.

Your optometrist shines a light in your eye and notices

Your optometrist shines a light in your eye and notices your pupil constricts in response to light. She tells you the iris is a muscle that adjust the sizes of the pupil automatically is response to light entering the ey ...

A patient had abnormally low ch50 results ie abnormally low

A patient had abnormally low CH50 results (i.e., abnormally low lysis of antibody coated sheep red blood cells) would abnormally low levels of Bb explain the CH50 assay results? Why or Why not?

Question overview although it may sound like science 2520

Question: Overview: Although it may sound like science 2520, DNA and RNA technologies are already used in products today. In this discussion you will examine one specific product, organism, or technology in your initial ...

4,153,160 Questions Asked
13,132 Experts
2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Recent Questions

Ask Biology Expert