1) Which one is a protease inhibitor ?
a) DDT b) Thiourea c) PMSF d) SDS
2) A non specific protease is ?
a) Pronase b) Trypsin c) Chymotrypsin d) Glu-C
3) The Tandem mass analyzer not used in ESI source is ?
a) Triple quadrupole b) Ion trap c) Fourier Transform Ion Cyclotron Resonance MS d) TOF
4) Which is not a physical Map?
a) Chromosomal map b) Radiation Hybrid map c) Linkage map d) Sequence map
5) Gene finding or prediction is an important application of ?
a) Functional genomics b) Structural genomics c) Comparative genomics d) Proteomics
6) Sanger’s dideoxy nucleotide method is also called ?
a) Maxam gilbert method b)SAGE c)Dye terminator sequencing d)Chain termination method
7) Microarray technology evolved from ?
a) Western blotting b) Southern blotting c) Northern blotting d) SDS – PAGE ?
8) Whole genome shotgun sequencing was done in ?
a) Institute for Genomic Research (TIGR)
b) European Bioinformatics Institute (EBI)
c) DOE-Joint Genome Institute
d) National Center for Biotechnology Information (NCBI)
9) The initiation of Human genome project took place in ?
a) 1990 b) 1980 c) 1947 d) 1981
10) 2’,3’ – dideoxynucleotide triphosphates (ddNTPs ) molecules differ from deoxynucleotides by having a H atom attached at which position to the carbon rather than an OH group ?
a) 2’ b) 2’, 3’ c) 3’ d) None
11) The program or algorithm that can be used for peptide mass fingerprinting is ?
a) MS-tag
b) Sequest
c) Mascot
d) SALSA
12) The Microarray technique also called Capture array ?
a) Reverse Phase protein Microarray
b) Functional protein Microarray
c) Analytical Microarray
d) DNA Microarray
13) The BAC to BAC method for sequencing the human genome is a ?
a) Map based method
b) Word method
c) Distance based method
d) Fragment based method
14) Tumor classification is done by which type of Microarray type ?
a) Expression analysis
b) Protein microarray
c) DNA microarray
d) Comparative Genome Hybridization ( CGH )
15) All the steps are required for in-gel digestion except ?
a) Reduction and Alkylation
b) Destaining
c) Proteolytic cleavage
d) Ionization
16) Tandem Affinity Purification requires fusion of which tag to the target protein of interest ?
a) His tag
b) Streptavidin tag
c) Myc tag
d) TAP tag
17) Cyanine dyes that are commonly used fluorophores with microarray are Cy ?
a) 3 , 5 b) 2 , 3 c) 5 , 6 4) 5 , 2
18) The 20 KDa protein with 20 protons in a solution will have a m/z ratio of ?
a) 1001 b) 1002 c) 40 d) 20
19) Which method is used for analysis of gene expression pattern ?
a) Microarray
b) SAGE
c) Northern Blotting
d) Maxam Gilbert method
20) The methods used by molecular biologists to produce snapshot of m-RNA in a sample of interest in the form of small tags is ?
a) PAGE
b) Reverse Transcription
c) Microarray
d) SAGE