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1. What is the correct stoichiometry for the complex formed when FGF-2 ginds and activates the FGF cell-surface receptor (FGFR)?

a. 1 molecule FGF-2: 1 FGFR

b. 1 molecule FGF-2:2 FGFR

c. 1 molecule FGF-2: 1FGFR: 1 heparan sulfate chain

d. 2 molecules FGF-2: 2FGFR: 2 heparan sulfate chain

e. 2 molecules FGF-2: 2FGFR: 1 heparan sulfate chain

2. Treatment with FGF-2 produces three different effects in NIH 3T3 cells. List these three effects. Which of these three effects are inhivited by the compound suramin?

3. What two signal transduction pathways are activated by FGF-2 binding to the DFGF receptor on NIH 3T3 CELLS? (General name)

How will you detect signaling down these two pathways? (what experiments will you do?)

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