1. What do you predict will happen to pulse amplitude when you raise your hand? lower your hand?

2. What can you conclude about the effect of altering hand position on pulse amplitude?

3. Based on the results of the experiment what types of experiments might you do in the future to investigate what other factors may affect pulse amplitude?

4. Explain the differences in cell size you observed under the microscope for cells in the 5% NaCl and 5% glucose.

5. Which solutions would be rated as:
Hyperosmotic

Hyposmotic

Isosmotic

4. Identify two mechanisms that could lead to fluid accumulation in a tissue or "edema" (movement of water from the blood compartment to the tissue compartment).

5. Discuss the relationship between osmotic gradient and the degree of hemolysis.

6. What effect did the addition of a local anesthetic have on RBC fragility?

7. How might local anesthetics exert their effects on RBCs?

8. What experiment would you perform next to address any questions that arose from this experiment?

9. What factors are responsible for creating an action potential in a neuron?

10. What is the cause for the relative and the absolute refractory periods?

11. Action potentials are said to be "all or none" responses. Why does the frog sciatic nerve give a graded response?

12. How do your results compare with the published conduction velocity of frog sciatic nerve?