1. How did the colonies from the two treated conditions different (shape, color, quantity, etc)?

2. For the bacteria which survived the heating, how did they do so?

3. What effect does the heating and cooling procedure have on the bacteria present in the soil samples?

4. After heating, which did you see more of? Gram positive or Gram negative bacteria?

5. Does heat effect bacteria with endospores?

6. What conditions can favor the production of endospores?

7. What would you conclude if all the samples were catalase negative?

8. Why is catalase such an important enzyme for bacteria?

9. In the Winogradsky column, where did the carbon source come from in the experimentation set up?

10. In general, why is carbon so important?

11. Why is the hydrogen sulfide gradient generated from the bottom to the top of the Winogradsky column?