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1. EMB media contains lactose and a small amount of sucrose. When making the media, you accidentally add excess sucrose so that there are equal levels of lactose and sucrose. Describe what you would observe on this plate compared to standard EMB media.

2. You perform the anal isolation and obtain single colonies on the EMB agar plate. From this you (CAN/CANNOT) conclude that you have a pure culture. Explain.

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